Biodegradation

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    Biodegradation is the biochemical process of the decomposition and mineralization of organic compounds that occurs, when organisms like bacteria, fungi, protozoa and other organisms use an organic substance as a source of carbon. It is a key phenomenon among environmental processes with the aim to assess a persistence  of organic substances.

    Through this biologically natural process, hazardous toxic contaminants are transformed into less toxic or non-toxic substances, resp. a change appears from complex substances into simpler ones before returning to the environment.... Read more...

    Ready Biodegradability

    A classification of biodegradability made by the OECD describing the degradation of an organic substance under aerobic conditions to carbon dioxide (CO2), water (H2O), and minerals by aerobic bacteria as determined by the measured change of Dissolved Organic Carbon (DOC), Biological Oxygen Demand (BOD), or CO2 evolution over time. A passing substance has to reach either a 60% BOD or theoretical CO2 evolution, or 70% decrease in DOC, depending on test method, all of which use a 10 - day window within a maximum 28-day test period for a successful determination. Readily biodegradable is defined as well as the ability of a product to biodegrade quickly and completely.

    GuidelineDescriptionSubstance properties
    OECD 301ADOC-Die-Away Test
    water soluble, not volatile
    OECD 301B
    CO2-Evolution Test (Sturm Test)
    insoluble in water, not volatile
    OECD 301D
    Closed-Bottle Test
    poor water soluble, volatile
    OECD 301F
    Manometric Respirometry Test
    insoluble in water, volatile
    OECD 310
    CO2-Headspace Test
    volatile

    The following table shows the different Biodegradation Tests according OECD 301 which are applied in dependence of the substance properties.

     Test     Analytical methodSuitability for compounds which are:
    poorly solublevolatile
    adsorbing
    DOC Die-Away (301A)
    Dissolved organic carbon
    --+/-
    Co2 Evolution (301B)Respirometry: CO2 evolution
    +-+
    Closed Bottle (301D)
    Respirometry: dissolved oxygen
    +/-++
    Manometric Respirometry (301F)
    Oxygen consumption
    ++/-+

    Inherent Biodegradability

    Inherent biodegradability refers to tests with a longer exposure of a test substance to microorganisms. Using favourable conditions, the tests of inherent biodegradability have been designed to assess whether the chemical has any potential for biodegradation under aerobic conditions. Inherently biodegradable is defined as the characteristic of a product to be consumed by naturally occurring organisms in nature over an indefinite period of time (> 20% but < 60% as measured by ready biodegradability testing). 

    GuidelineDescriptionSubstance properties
    OECD 302BZahn-Wellens / EMPA Test
    insoluble in water, not volatile, filterable

    Special Biodegradation Tests

    InterBiotox offers also special biodegradation studies in anaerobic conditions simulating the conditions similar to an anaerobic digester. The other biodegradation studies simulates the biotic degradation of plastic materials in an aqueous medium.

    GuidelineDescription
    OECD 311/ISO 11734Anaerobic Biodegradability of Organic Compounds in Digested Sludge
    EN ISO 14851:2019
    Determination of the ultimate aerobic biodegradability of plastic materials in an aqueous medium - Method by measuring the oxygen demand in a closed respirometer
    EN ISO 14852:2018
    Determination of the ultimate aerobic biodegradability of plastic materials in an aqueous medium - Method by analysis of evolved carbon dioxide
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